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验证数据展示

  • WB analysis of HEK-293 using 67372-1-Ig

    WB analysis of HEK-293 using 67372-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67372-1-Ig

    WB analysis of HepG2 using 67372-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67372-1-Ig

    IHC staining of human breast cancer using 67372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH9A1 antibody included
  • IHC staining of human breast cancer using 67372-1-Ig

    IHC staining of human breast cancer using 67372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH9A1 antibody included

ALDH9A1 Monoclonal antibody

ALDH9A1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67372-1-Ig

产品说明书

CloneNo. 3C5C9

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

ALDH4, ALDH7, ALDH9, ALDH9A1, E3, TMABADH

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALDH9A1 Ready-To-Use IHC Kit. ALDH9A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67372-1-Ig targets ALDH9A1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag24493

Product name: Recombinant human ALDH9A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 285-412 aa of BC151140

Sequence: LIIFSDCDMNNAVKGALMANFLTQGQVCCNGTRVFVQKEILDKFTEEVVKQTQRIKIGDPLLEDTRMGPLINRPHLERVLGFVKVAKEQGAKVLCGGDIYVPEDPKLKDGYYMRPCVLTNCRDDMTCV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 9 family, member A1
别名 ALDH4, ALDH7, ALDH9, ALDH9A1, E3, TMABADH
计算分子量 518 aa, 56 kDa
观测分子量 46 kDa
GenBank蛋白编号BC151140
基因名称 ALDH9A1
Gene ID (NCBI) 223
RRIDAB_2882620
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP49189
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.

实验方案

Product Specific Protocols
IHC protocol for ALDH9A1 antibody 67372-1-IgDownload protocol
WB protocol for ALDH9A1 antibody 67372-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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