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验证数据展示

  • IF Staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

    IF Staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ALDH5A1 antibody (67347-1-Ig, Clone: 1B9H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

  • WB analysis of L02 using 67347-1-Ig (same clone as 67347-1-PBS)

    WB analysis of L02 using 67347-1-Ig (same clone as 67347-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67347-1-Ig (same clone as 67347-1-PBS)

    WB analysis of HepG2 using 67347-1-Ig (same clone as 67347-1-PBS)

    WB result of ALDH5A1 antibody (67347-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

    IHC staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

    IHC staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 67347-1-Ig (same clone as 67347-1-PBS)

    FC experiment of A431 using 67347-1-Ig (same clone as 67347-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ALDH5A1 (67347-1-Ig, Clone:1B9H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67347-1-Ig (same clone as 67347-1-PBS)

    IF Staining of mouse liver using 67347-1-Ig (same clone as 67347-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ALDH5A1 antibody (67347-1-Ig, Clone: 1B9H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ALDH5A1 Monoclonal antibody, PBS Only

ALDH5A1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 67347-1-PBS

产品说明书

CloneNo. 1B9H10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

Succinate-semialdehyde dehydrogenase, mitochondrial, SSDH, SSADH, NAD(+)-dependent succinic semialdehyde dehydrogenase, EC:1.2.1.24

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67347-1-PBS targets ALDH5A1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag11155

Product name: Recombinant human ALDH5A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 175-535 aa of BC034321

Sequence: YGDIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAMITRKVGAALAAGCTVVVKPAEDTPFSALALAELASQAGIPSGVYNVIPCSRKNAKEVGEAICTDPLVSKISFTGSTTTGKILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVAGAMASKFRNTGQTCVCSNQFLVQRGIHDAFVKAFAEAMKKNLRVGNGFEEGTTQGPLINEKAVEKVEKQVNDAVSKGATVVTGGKRHQLGKNFFEPTLLCNVTQDMLCTHEETFGPLAPVIKFDTEEEAIAIANAADVGLAGYFYSQDPAQIWRVAEQLEVGMVGVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLELKYVCYGGL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 5 family, member A1
别名 Succinate-semialdehyde dehydrogenase, mitochondrial, SSDH, SSADH, NAD(+)-dependent succinic semialdehyde dehydrogenase, EC:1.2.1.24
计算分子量 535 aa, 57 kDa
观测分子量 51 kDa
GenBank蛋白编号BC034321
基因名称 ALDH5A1
Gene ID (NCBI) 7915
RRIDAB_2882605
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP51649
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C