验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, HepG2 cells, human brain tissue, human liver tissue, human skeletal muscle tissue, K-562 cells, mouse skeletal muscle tissue, mouse small intestine tissue, rat heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ALDH4A1 Ready-To-Use IHC Kit. ALDH4A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
11604-1-AP targets ALDH4A1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ALDH4A1 fusion protein Ag2147 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aldehyde dehydrogenase 4 family, member A1 |
| 别名 | ALDH4, ALDH4A1, P5C dehydrogenase, P5CD, P5CDh, P5CDhL, P5CDhS |
| 计算分子量 | 563 aa, 62 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC023600 |
| 基因名称 | ALDH4A1 |
| Gene ID (NCBI) | 8659 |
| RRID | AB_2258046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30038 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate.Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH4A1 antibody 11604-1-AP | Download protocol |
| IHC protocol for ALDH4A1 antibody 11604-1-AP | Download protocol |
| IP protocol for ALDH4A1 antibody 11604-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.
| ||
Sci Rep Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure. | ||
J Exp Clin Cancer Res Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer. | ||
bioRxiv Mitochondrial NADK2-dependent NADPH controls Tau oligomer uptake in human neurons | ||
Nat Metab Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells
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