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验证数据展示

  • WB analysis using 21391-1-AP (same clone as 21391-1-PBS)

    WB analysis using 21391-1-AP (same clone as 21391-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21391-1-AP (ALDH1L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21391-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 21391-1-AP (same clone as 21391-1-PBS)

    IHC staining of human pancreas using 21391-1-AP (same clone as 21391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21391-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1L2 antibody included
  • IHC staining of human pancreas using 21391-1-AP (same clone as 21391-1-PBS)

    IHC staining of human pancreas using 21391-1-AP (same clone as 21391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21391-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1L2 antibody included
  • IF Staining of A431 using 21391-1-AP (same clone as 21391-1-PBS)

    IF Staining of A431 using 21391-1-AP (same clone as 21391-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ALDH1L2 antibody (21391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21391-1-PBS in a different storage buffer formulation.

  • WB analysis using 21391-1-AP (same clone as 21391-1-PBS)

    WB analysis using 21391-1-AP (same clone as 21391-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21391-1-AP (ALDH1L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21391-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ALDH1L2 Polyclonal antibody, PBS Only

ALDH1L2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21391-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ALDH1L2 Ready-To-Use IHC Kit. ALDH1L2 primary antibody included.

产品信息

21391-1-PBS targets ALDH1L2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16046

Product name: Recombinant human ALDH1L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-103 aa of BC103935

Sequence: MVTFYGSTLLNSSVPPGEPLEIKGAKKPGLVTKNGLVLFGNDGKALTVRNLQFEDGKMIPASQYFSTGETSVVELTAEEVKVAETIKVIWAGILSNVPIIEDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 1 family, member L2
别名
计算分子量 923 aa, 102 kDa
观测分子量 102 kDa, 89 kDa
GenBank蛋白编号BC103935
基因名称 ALDH1L2
Gene ID (NCBI) 160428
RRIDAB_2878854
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3SY69
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALDH1L2(Aldehyde dehydrogenase family 1 member L2) is also named as mitochondrial 10-FTHFDH, mtFDH and belongs to the aldehyde dehydrogenase family and ALDH1L subfamily. The ALDH1L2 gene has three transcriptional variants with the molecular weight of 102 kDa, 42 kDa and 89 kDa(PMID:19823103). It is a mitochondrial enzyme, a likely source of CO2 production from 10-formyltetrahydrofolate in mitochondria and playing an essential role in the distribution of one-carbon groups between the cytosolic and mitochondrial compartments of the cell(PMID: 20498374). It also has dehydrogenase/hydrolase activities similar to cytosolic FDH (ALDH1L1). The native ALDH1L2 has been reported to exist as a dimer or tetramer(PMID:7822273).

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