ALDH1B1 Polyclonal antibody
ALDH1B1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, rat brain tissue |
Positive IHC detected in | human testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
The immunogen of 22220-1-AP is ALDH1B1 Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC, IHC, WB,ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat |
Immunogen | ALDH1B1 fusion protein Ag17557 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | aldehyde dehydrogenase 1 family, member B1 |
Synonyms | Aldehyde dehydrogenase 5, ALDH1B1, ALDH5, ALDHX |
Calculated Molecular Weight | 517 aa, 57 kDa |
Observed Molecular Weight | 55-58 kDa |
GenBank Accession Number | BC001619 |
Gene Symbol | ALDH1B1 |
Gene ID (NCBI) | 219 |
RRID | AB_2879035 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P30837 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ALDH1B1 antibody 22220-1-AP | Download protocol |
IHC protocol for ALDH1B1 antibody 22220-1-AP | Download protocol |
IF protocol for ALDH1B1 antibody 22220-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncotarget Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats. | ||
Transl Cancer Res ALDH1B1 predicts poor survival for locally advanced nasopharyngeal carcinoma patients. |