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Cardinal Red™ Anti-Human ALCAM (3A6)

ALCAM Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

3A6

Cat No : CR-65223

Print datasheet

Synonyms

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

验证数据展示

  • FC experiment of human PBMCs using CR-65223

    FC experiment of human PBMCs using CR-65223

    1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human ALCAM (CR-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5 ul Cardinal Red™ Anti-Human ALCAM (CR-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inPHA-treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-65223 targets ALCAM in FC applications and shows reactivity with Human samples.

Tested Applications FC
Tested Reactivity Human
Immunogen Cultured human thymic epithelial cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name activated leukocyte cell adhesion molecule
Synonyms ALCAM, CD166, CD166 antigen, FLJ38514, MEMD
Calculated Molecular Weight 105 kDa
GenBank Accession NumberBC057809
Gene Symbol ALCAM
Gene ID (NCBI) 214
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ13740
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

实验方案

Product Specific Protocols
FC protocol for Cardinal Red™ ALCAM antibody CR-65223Download protocol
Standard Protocols
Click here to view our Standard Protocols