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验证数据展示

  • WB analysis of mouse lung using 84528-1-RR (same clone as 84528-1-PBS)

    WB analysis of mouse lung using 84528-1-RR (same clone as 84528-1-PBS)

    mouse lung tissue was subjected to SDS PAGE followed by western blot with 84528-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01379-1

    Sandwich ELISA standard curve of MP01379-1

    Sandwich ELISA standard curve of MP01379-1, Mouse ALCAM Recombinant Matched Antibody Pair - PBS only. 84528-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1524. 84528-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01379-1

    Sample test of MP01379-1

    Serum of six mice was measured. The ALCAM concentration of detected samples was determined to be 43,380.5 pg/mL with a range of 28,091.3-50,380.4 pg/mL.

  • Sample test of MP01379-1

    Sample test of MP01379-1

    The mean ALCAM concentration was determined to be 888,916.9 pg/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load and 518,424.5 pg/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP01379-1

    Cytometric bead array standard curve of MP01379-1

    Cytometric bead array standard curve of MP01379-1, MOUSE ALCAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84528-1-PBS. Detection antibody: 84528-3-PBS. Standard: Eg1524. Range: 0.781-100 ng/mL.

Mouse ALCAM Recombinant antibody, PBS Only (Capture)

ALCAM Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84528-1-PBS

产品说明书

CloneNo. 241945A4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

241945A4, Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84528-1-PBS targets ALCAM as part of a matched antibody pair:

MP01379-1: 84528-1-PBS capture and 84528-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 ALCAM fusion protein Eg1524 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activated leukocyte cell adhesion molecule
别名 241945A4, Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen
计算分子量65 kDa
观测分子量90-100 kDa
GenBank蛋白编号NM_009655.3
基因名称 Alcam
Gene ID (NCBI) 11658
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ61490
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).