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验证数据展示

  • WB analysis using 32402-1-AP

    WB analysis using 32402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32402-1-AP (ALCAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 32402-1-AP

    IHC staining of mouse liver using 32402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 32402-1-AP

    IHC staining of mouse liver using 32402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ALCAM Polyclonal antibody

ALCAM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32402-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen, Protein DM-GRASP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse lung tissue, rat liver tissue
Positive IHC detected inmouse liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32402-1-AP targets ALCAM in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activated leukocyte cell adhesion molecule
别名 Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen, Protein DM-GRASP
计算分子量65 kDa
观测分子量90-110 kDa
GenBank蛋白编号NM_009655.3
基因名称 Alcam
Gene ID (NCBI) 11658
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ61490
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

实验方案

Product Specific Protocols
WB protocol for ALCAM antibody 32402-1-APDownload protocol
IHC protocol for ALCAM antibody 32402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols