验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, pig adrenal gland tissue, rat adrenal gland tissue, A549 cells, HepG2 cells, Jurkat cells, K-562 cells, human adrenal gland tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68567-1-Ig targets ALAS1 in WB, ELISA applications and shows reactivity with Human, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, rat, pig |
| 免疫原 |
CatNo: Ag33580 Product name: Recombinant human ALAS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-330 aa of BC011798 Sequence: MESVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRALSTAAVHYQQIKETPPASEKDKTAKAKVQQTPDGSQQSPDGTQLPSGHPLPATSQGTASKCPFLAAQMNQRGSSVFCKASLELQEDVQEMNAVRKEVAETSAGPSVVSVKTDGGDPSGLLKNFQDIMQKQRPERVSHLLQDNLPKSVSTFQYDRFFEKKIDEKKNDHTYRVFKTVNRRAHIFPMADDYSDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLERELADLHGKDAALLFSSCFVANDSTLFTLAKMMPGC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aminolevulinate, delta-, synthase 1 |
| 别名 | ALAS, ALAS H, ALAS1, ALAS3, ALASH, Delta ALA synthase 1, MIG4 |
| 计算分子量 | 640 aa, 71 kDa |
| 观测分子量 | 68-70 kDa |
| GenBank蛋白编号 | BC011798 |
| 基因名称 | ALAS1 |
| Gene ID (NCBI) | 211 |
| RRID | AB_3085269 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P13196 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALAS1 antibody 68567-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
