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验证数据展示

  • WB analysis using 31686-1-AP (same clone as 31686-1-PBS)

    WB analysis using 31686-1-AP (same clone as 31686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31686-1-AP (ALAD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31686-1-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 31686-1-AP (same clone as 31686-1-PBS)

    WB analysis of U-251 using 31686-1-AP (same clone as 31686-1-PBS)

    WB result of ALAD antibody (31686-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALAD transfected U-251 cells. This data was developed using the same antibody clone with 31686-1-PBS in a different storage buffer formulation.

ALAD Polyclonal antibody, PBS Only

ALAD Polyclonal Antibody for WB, Indirect ELISA
Cat No. 31686-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

ALADH, Delta aminolevulinic acid dehydratase, Porphobilinogen synthase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31686-1-PBS targets ALAD in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35649

Product name: Recombinant human ALAD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 151-330 aa of NM_000031.5

Sequence: AEVALAYAKAGCQVVAPSDMMDGRVEAIKEALMAHGLGNRVSVMSYSAKFASCFYGPFRDAAKSSPAFGDRRCYQLPPGARGLALRAVDRDVREGADMLMVKPGMPYLDIVREVKDKHPDLPLAVYHVSGEFAMLWHGAQAGAFDLKAAVLEAMTAFRRAGADIIITYYTPQLLQWLKEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aminolevulinate, delta-, dehydratase
别名 ALADH, Delta aminolevulinic acid dehydratase, Porphobilinogen synthase
计算分子量36kDa，330aa
观测分子量 36-39 kDa
GenBank蛋白编号NM_000031.5
基因名称 ALAD
Gene ID (NCBI) 210
RRIDAB_3670079
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP13716
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Delta-aminolevulinic acid dehydratase (ALAD) is also named as Porphobilinogen synthase. ALAD, encoded by the moonlighting gene ALAD, not only participates in the second step of heme biosynthesis, but also inhibits the 26S proteasome (PMID: 35318110). ALAD is involved in tumor progression. For example, ALAD2 allele (ALAD polymorphism) is related to an increased risk for meningioma, and downregulation of ALAD is related to poor prognosis of patients with breast cancer (PMID: 16140629, PMID: 28403546).

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