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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85769-4-RR (same clone as 85769-4-PBS)

    WB analysis using 85769-4-RR (same clone as 85769-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85769-4-RR (AKT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85769-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85769-4-RR

    Affinity and Kinetic Characterization of 85769-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85769-4-RR against Human AKT3 were performed. The affinity constant is 0.696 nM.

AKT3 Recombinant antibody, PBS Only

AKT3 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85769-4-PBS

产品说明书

CloneNo. 250046E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

EC:2.7.11.1, PKB gamma, PKBG, PRKBG, Protein kinase Akt 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85769-4-PBS targets AKT3 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AKT3 fusion protein Ag16298 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
别名 EC:2.7.11.1, PKB gamma, PKBG, PRKBG, Protein kinase Akt 3
计算分子量 465 aa, 54 kDa
观测分子量 60 kDa
GenBank蛋白编号BC121154
基因名称 AKT3
Gene ID (NCBI) 10000
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y243
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKT3, also named as PKBG, is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas.