Phospho-AKT (Ser473) Polyclonal antibody

Phospho-AKT (Ser473) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28731-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IF/ICC, ELISA and More (2)

AKT (Ser473), p AKT, p AKT (Ser473), p-AKT, p-AKT (Ser473)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inCalyculin A treated PC-3 cells, PDGF treated NIH/3T3 cells
Positive IF/ICC detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28731-1-AP targets Phospho-AKT (Ser473) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, rabbit, sheep
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 1
别名 AKT (Ser473), p AKT, p AKT (Ser473), p-AKT, p-AKT (Ser473)
观测分子量 58 kDa
GenBank蛋白编号NM_005163
基因名称 AKT1
Gene ID (NCBI) 207
RRIDAB_2881201
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31749
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA (PubMed:20333297). This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation (PubMed:9512493). Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1 (PubMed:21464307, PubMed:8978681). AKT1 Thr-308 and Ser-473 is Phosphorylated by IKBKE and TBK1 (PubMed:15718470, PubMed:18456494, PubMed:20481595, PubMed:8978681). Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A) (PubMed:14761976). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells (PubMed:17013611). Ser-473 phosphorylation is enhanced by signaling through activated FLT3 (By similarity). Ser-473 is dephosphorylated by PHLPP (PubMed:28147277) and Ser-473 by PP2A phosphatase (PubMed:21329884).

实验方案

Product Specific Protocols
WB protocol for Phospho-AKT (Ser473) antibody 28731-1-APDownload protocol
IF protocol for Phospho-AKT (Ser473) antibody 28731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Toxicology

Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice

Authors - Yong-Jiang Zhou
mouseWB

J Neuroinflammation

TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis

Authors - Lin Wang
mouseWB

Phytomedicine

Combinational application of the natural products 1-deoxynojirimycin and morin ameliorates insulin resistance and lipid accumulation in prediabetic mice

Authors - Xinxiu Ren
humanWB

Biomed Pharmacother

Preclinical characterization of danatinib as a novel FLT3 inhibitor with excellent efficacy against resistant acute myeloid leukemia

Authors - Shan-Liang Sun
humanWB

Cell Biosci

Serine protease PRSS56, a novel cancer-testis antigen activated by DNA hypomethylation, promotes colorectal and gastric cancer progression via PI3K/AKT axis

Authors - Dandan Li
ratWB

Biomed Pharmacother

Cinnamaldehyde protects donor heart from cold ischemia-reperfusion injury via the PI3K/AKT/mTOR pathway

Authors - Hongwen Lan
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