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验证数据展示

  • WB analysis of mouse liver using 10730-1-AP

    WB analysis of mouse liver using 10730-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10730-1-AP

    WB analysis of HEK-293 using 10730-1-AP

    WB result of AKR7A2 antibody (10730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells.

  • IHC staining of human liver cancer using 10730-1-AP

    IHC staining of human liver cancer using 10730-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10730-1-AP (AKR7A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKR7A2 antibody included
  • IHC staining of human liver cancer using 10730-1-AP

    IHC staining of human liver cancer using 10730-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10730-1-AP (AKR7A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKR7A2 antibody included
  • IHC staining of human testis using 10730-1-AP

    IHC staining of human testis using 10730-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10730-1-AP (AKR7A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKR7A2 antibody included
  • IHC staining of mouse kidney using 10730-1-AP

    IHC staining of mouse kidney using 10730-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10730-1-AP (AKR7A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKR7A2 antibody included
  • IHC staining of mouse liver using 10730-1-AP

    IHC staining of mouse liver using 10730-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10730-1-AP (AKR7A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKR7A2 antibody included
  • Featured Product
  • KD/KO Validated

AKR7A2 Polyclonal antibody

AKR7A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10730-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

AFAR, AFB1 AR 1, AFB1 AR1, AFB1-AR 1, AKR7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, A431 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® AKR7A2 Ready-To-Use IHC Kit. AKR7A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10730-1-AP targets AKR7A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag1049

Product name: Recombinant human AKR7A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 60-359 aa of BC007352

Sequence: RAFLERGHTELDTAFMYSDGQSETILGGLGLGLGGGDCRVKIATKANPWDGKSLKPDSVRSQLETSLKRLQCPQVDLFYLHAPDHGTPVEETLHACQRLHQEGKFVELGLSNYASWEVAEICTLCKSNGWILPTVYQGMYNATTRQVETELFPCLRHFGLRFYAYNPLAGGLLTGKYKYEDKDGKQPVGRFFGNSWAETYRNRFWKEHHFEAIALVEKALQAAYGASAPSVTSAALRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAATEEGPLEPAVVDAFNQAWHLVAHECPNYFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
别名 AFAR, AFB1 AR 1, AFB1 AR1, AFB1-AR 1, AKR7
计算分子量 40 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC007352
基因名称 AKR7A2
Gene ID (NCBI) 8574
RRIDAB_2224439
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43488
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells (PMID:22001351).

实验方案

Product Specific Protocols
IHC protocol for AKR7A2 antibody 10730-1-APDownload protocol
WB protocol for AKR7A2 antibody 10730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Environ Toxicol

Naringin alleviates acetaminophen-induced acute liver injury by activating Nrf2 via CHAC2 upregulation.

Authors - Xiaohan Zhai
View Article
humanWB,IHC

Pancreatology

Proteomic analysis of pancreatic ductal adenocarcinoma compared with normal adjacent pancreatic tissue and pancreatic benign cystadenoma.

Authors - Cui Yazhou Y
View Article
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