验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23910-1-AP targets AKR1D1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag21009 Product name: Recombinant human AKR1D1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 209-326 aa of BC130627 Sequence: CQQHDIVITAYSPLGTSRNPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) |
| 别名 | 3o5bred, AKR1D1, CBAS2, SRD5B1 |
| 计算分子量 | 326 aa, 37 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC130627 |
| 基因名称 | AKR1D1 |
| Gene ID (NCBI) | 6718 |
| RRID | AB_2879359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P51857 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKR1D1 (steroid 5β-reductase) reduces all Δ 4-3-ketosteroids to form 5β-dihydrosteroids, a first step in the clearance of steroid hormones and an essential step in the synthesis of all bile acids. It is also named as SRD5B1 and belongs to the aldo/keto reductase family. This protein has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AKR1D1 antibody 23910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
