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AKR1CL2 Polyclonal antibody

AKR1CL2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20525-1-AP

Print datasheet

Synonyms

AF reductase, AKR1CL2, AKR1E2, AKRDC1, hTSP, LoopADR, Testis specific protein

验证数据展示

  • WB analysis of mouse testis using 20525-1-AP

    WB analysis of mouse testis using 20525-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20525-1-AP

    IF Staining of HeLa using 20525-1-AP

    Immunofluorescent analysis of Hela cells, using AKR1CL2 antibody 20525-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse testis tissue, HT-1080 cells
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20525-1-AP is AKR1CL2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen AKR1CL2 fusion protein Ag14381 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldo-keto reductase family 1, member C-like 2
Synonyms AF reductase, AKR1CL2, AKR1E2, AKRDC1, hTSP, LoopADR, Testis specific protein
Calculated Molecular Weight 320 aa, 37 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC002862
Gene Symbol AKR1CL2
Gene ID (NCBI) 83592
RRIDAB_10694285
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKR1CL2 antibody 20525-1-APDownload protocol
IF protocol for AKR1CL2 antibody 20525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols