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验证数据展示

  • WB analysis using 18852-1-AP

    WB analysis using 18852-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18852-1-AP (AKR1C4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

AKR1C4 Polyclonal antibody

AKR1C4 Polyclonal Antibody for WB, ELISA
Cat No. 18852-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA and More (1)

别名

CHDR, CDR, AKR1C 4, 3-alpha-hydroxysteroid dehydrogenase type I, 3alpha-hydroxysteroid 3-dehydrogenase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18852-1-AP targets AKR1C4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag13444

Product name: Recombinant human AKR1C4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC020744

Sequence: MDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNYRQLEMILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRENIQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4)
别名 CHDR, CDR, AKR1C 4, 3-alpha-hydroxysteroid dehydrogenase type I, 3alpha-hydroxysteroid 3-dehydrogenase
计算分子量 323 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC020744
基因名称 AKR1C4
Gene ID (NCBI) 1109
RRIDAB_10665359
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17516
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKR1C4 antibody 18852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Redox Biol

Ubiquitination as a key regulatory mechanism for O3-induced cutaneous redox inflammasome activation

Authors - Francesca Ferrara
View Article