×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using RMX00069

    WB analysis using RMX00069

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00069 (AKR1C3 antibody) at dilution of 1:17000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using RMX00069

    IHC staining of human breast cancer using RMX00069

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using RMX00069 (AKR1C3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using RMX00069

    IF Staining of A549 using RMX00069

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using AKR1C3 antibody (RMX00069) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® AKR1C3 Multi-Recombinant antibody

AKR1C3 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00069

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, rat kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00069 targets AKR1C3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
别名 17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2
计算分子量 323 aa, 37 kDa
观测分子量 34 kDa
GenBank蛋白编号BC019230
基因名称 AKR1C3
Gene ID (NCBI) 8644
RRIDAB_3750118
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP42330
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism.

实验方案

Product Specific Protocols
IF protocol for AKR1C3 antibody RMX00069Download protocol
IHC protocol for AKR1C3 antibody RMX00069Download protocol
WB protocol for AKR1C3 antibody RMX00069Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...