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验证数据展示

  • WB analysis using 84872-5-RR

    WB analysis using 84872-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84872-5-RR (AKR1C3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 84872-5-RR

    IHC staining of human prostate cancer using 84872-5-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84872-5-RR (AKR1C3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84872-5-RR

    Affinity and Kinetic Characterization of 84872-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84872-5-RR against Human AKR1C3 were performed. The affinity constant is 35.8 nM.

AKR1C3 Recombinant antibody

AKR1C3 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84872-5-RR

产品说明书

CloneNo. 242222A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 242222A11, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, A549 cells, HepG2 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84872-5-RR targets AKR1C3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AKR1C3 fusion protein Ag1674 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
别名 17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 242222A11, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain
计算分子量 323 aa, 37 kDa
观测分子量35 kDa
GenBank蛋白编号BC019230
基因名称 AKR1C3
Gene ID (NCBI) 8644
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42330
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism.

实验方案

Product Specific Protocols
WB protocol for AKR1C3 antibody 84872-5-RRDownload protocol
IHC protocol for AKR1C3 antibody 84872-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols