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验证数据展示

  • WB analysis using 84872-5-RR (same clone as 84872-5-PBS)

    WB analysis using 84872-5-RR (same clone as 84872-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84872-5-RR (AKR1C3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84872-5-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 84872-5-RR (same clone as 84872-5-PBS)

    IHC staining of human prostate cancer using 84872-5-RR (same clone as 84872-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84872-5-RR (AKR1C3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84872-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84872-5-RR (same clone as 84872-5-PBS)

    IF Staining of HepG2 using 84872-5-RR (same clone as 84872-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AKR1C3 antibody (84872-5-RR, Clone: 242222A11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84872-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84872-5-RR

    Affinity and Kinetic Characterization of 84872-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84872-5-RR against Human AKR1C3 were performed. The affinity constant is 35.8 nM.

AKR1C3 Recombinant monoclonal antibody, PBS Only

AKR1C3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84872-5-PBS

产品说明书

CloneNo. 242222A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 242222A11, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84872-5-PBS targets AKR1C3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1674

Product name: Recombinant human AKR1C3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC019230

Sequence: MDSKHQCVKLNDGHFMPVLGFGTYAPPEVPRSKALEVTKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSTFHRPELVRPALENSLKKAQLDYVDLYLIHSPMSLKPGEELSPTDENGKVIFDIVDLCTTWEAMEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNRSKLLDFCKSKDIVLVAYSALGSQRDKRWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTAEDMKAIDGLDRNLHYFNSDSFASHPNYPYSDEY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
别名 17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 242222A11, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain
计算分子量 323 aa, 37 kDa
观测分子量35 kDa
GenBank蛋白编号BC019230
基因名称 AKR1C3
Gene ID (NCBI) 8644
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42330
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism.

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