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AKR1B10 Polyclonal antibody

AKR1B10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 18252-1-AP

Print datasheet

Synonyms

AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HSI, SI reductase, Small intestine reductase

验证数据展示

  • WB analysis using 18252-1-AP

    WB analysis using 18252-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 18252-1-AP

    WB analysis using 18252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, mouse liver tissue, HepG2 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy AKR1B10 Ready-To-Use IHC Kit. AKR1B10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18252-1-AP targets AKR1B10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AKR1B10 fusion protein Ag13042 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldo-keto reductase family 1, member B10 (aldose reductase)
Synonyms AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HSI, SI reductase, Small intestine reductase
Calculated Molecular Weight 316 aa, 36 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC008837
Gene Symbol AKR1B10
Gene ID (NCBI) 57016
RRIDAB_2878524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60218
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKR1B10 antibody 18252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Pharmacol Exp Ther

Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.

Authors - Ramon Martinez
View Article