验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, L02 cells, human liver tissue, BxPC-3 cells, HepG2 cells, human placenta tissue |
| Positive IF/ICC detected in | BxPC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy AKR1B1 Ready-To-Use IHC Kit. AKR1B1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 12 publications below |
| IHC | See 2 publications below |
产品信息
15439-1-AP targets AKR1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag7142 Product name: Recombinant human AKR1B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC000260 Sequence: MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQNENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDLKLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEELVDEGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYCQSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLIRFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCALLSCTSHKDYPFHEEF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aldo-keto reductase family 1, member B1 (aldose reductase) |
| 别名 | AKR1B 1, Aldo-keto reductase family 1 member B1, ALDR1, ALR2, AR |
| 计算分子量 | 36 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC000260 |
| 基因名称 | AKR1B1 |
| Gene ID (NCBI) | 231 |
| RRID | AB_2224154 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15121 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AKR1B1 antibody 15439-1-AP | Download protocol |
| IF protocol for AKR1B1 antibody 15439-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling. | ||
Biochem Pharmacol Regulation on SIRT1-PGC-1α/Nrf2 pathway together with selective inhibition of aldose reductase makes compound hr5F a potential agent for the treatment of diabetic complications. | ||
Biomed Pharmacother Systematic investigation of the pharmacological mechanism for renal protection by the leaves of Eucommia ulmoides Oliver using UPLC-Q-TOF/MS combined with network pharmacology analysis. | ||
Toxicol Appl Pharmacol Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways. | ||
BMC Nephrol Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells. | ||
Fertil Steril Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation. |
