验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, mouse lung tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy AKR1A1 Ready-To-Use IHC Kit. AKR1A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15054-1-AP targets AKR1A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | AKR1A1 fusion protein Ag7037 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aldo-keto reductase family 1, member A1 (aldehyde reductase) |
| 别名 | EC:1.1.1.19, ALR, ALDR1, Aldo-keto reductase family 1 member A1, Alcohol dehydrogenase [NADP(+)] |
| 计算分子量 | 37 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC000670 |
| 基因名称 | AKR1A1 |
| Gene ID (NCBI) | 10327 |
| RRID | AB_2224138 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14550 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| IHC protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| IF protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| IP protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.
| ||
J Med Chem Identification of a Selective SCoR2 Inhibitor That Protects Against Acute Kidney Injury | ||