验证数据展示
经过测试的应用
| Positive IP detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22649-1-AP targets AKIRIN1 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | AKIRIN1 fusion protein Ag18530 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | akirin 1 |
| 别名 | akirin 1, C1orf108, RP11 781D11.2, STRF2 |
| 计算分子量 | 192 aa, 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC119745 |
| 基因名称 | AKIRIN1 |
| Gene ID (NCBI) | 79647 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H9L7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Akirin1 is a highly conserved ubiquitously expressed nuclear protein harboring a clear nuclear localization signal and the protein-protein interaction property. Acting as a cofactor, Akirin1 has been disclosed that regulate transcriptional activities by establishing a link between multiple transcription factors and chromatin remodeling complexes (PMID: 39188701) . Akirin1 has been shown to be involved in the induction of cardiac hypertrophy via miR-1 and serum response factor (SRF) in mice (PMID: 30746755).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for AKIRIN1 antibody 22649-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |