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验证数据展示

  • WB analysis using 27554-1-AP

    WB analysis using 27554-1-AP

    Various lysates lysate were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of SMMC-7721 using 27554-1-AP

    WB analysis of SMMC-7721 using 27554-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 27554-1-AP

    IHC staining of human heart using 27554-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 27554-1-AP

    IHC staining of human heart using 27554-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 40x lens).

AKD2 Polyclonal antibody

AKD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27554-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, IHC, ELISA

别名

Adenylate kinase 9, AK 9, AK9, AKD1, AKD2, C6orf199, C6orf224, RP1 70A9.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSMMC-7721 cells, HepG2 cells, HuH-7 cells
Positive IHC detected inhuman heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27554-1-AP targets AKD2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 AKD2 fusion protein Ag25805 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenylate kinase domain containing 2
别名 Adenylate kinase 9, AK 9, AK9, AKD1, AKD2, C6orf199, C6orf224, RP1 70A9.1
计算分子量221 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC022031
基因名称 AKD2
Gene ID (NCBI) 221264
RRIDAB_2880904
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TCS8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).

实验方案

Product Specific Protocols
WB protocol for AKD2 antibody 27554-1-APDownload protocol
IHC protocol for AKD2 antibody 27554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Carcinog

Integrative analyses of N6-methyladenosine-associated single-nucleotide polymorphisms (m6A-SNPs) identify tumor suppressor gene AK9 in lung cancer

Authors - Tingting Hua
View Article