验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HL-60 cells, HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31542-1-AP targets AKAP8 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag35816 Product name: Recombinant human AKAP8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 501-692 aa of NM_005858.3 Sequence: SVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | A kinase (PRKA) anchor protein 8 |
| 别名 | A kinase anchor protein 8, A kinase anchor protein 95 kDa, AKAP 8, AKAP 95, AKAP8, AKAP95, DKFZp586B1222 |
| 计算分子量 | 76kDa，692aa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | NM_005858.3 |
| 基因名称 | AKAP8 |
| Gene ID (NCBI) | 10270 |
| RRID | AB_3670031 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O43823 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKAP8 (A-kinase anchor protein 8), also called AKAP95, is a A-kinase anchor protein that directs activity of protein kinase A by tethering the enzyme near its physiologic substrates (PMID: 9473338; 10601332). It is associated with chromatin and nuclear matrix which has a remarkable activity in promoting chromatin transcription (PMID: 31980632), and it is also required for cell cycle G2/M transition and histone deacetylation during mitosis (PMID: 16980585).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AKAP8 antibody 31542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
