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验证数据展示

  • WB analysis of human brain using 12591-1-AP (same clone as 12591-1-PBS)

    WB analysis of human brain using 12591-1-AP (same clone as 12591-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12591-1-AP (same clone as 12591-1-PBS)

    IF Staining of HepG2 using 12591-1-AP (same clone as 12591-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using AKAP7 antibody 12591-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 12591-1-AP (same clone as 12591-1-PBS)

    IHC staining of human brain using 12591-1-AP (same clone as 12591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 12591-1-AP (same clone as 12591-1-PBS)

    IHC staining of human brain using 12591-1-AP (same clone as 12591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AKAP7 Polyclonal antibody, PBS Only

AKAP7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12591-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

A kinase anchor protein 18 kDa, AKAP 18, AKAP 7 isoforms alpha and beta, AKAP18, AKAP-7 isoforms alpha and beta

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12591-1-PBS targets AKAP7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3297

Product name: Recombinant human AKAP7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-81 aa of BC016927

Sequence: MGQLCCFPFSRDEGKISEKNGGEPDDAELVRLSKRLVENAVLKAVQQYLEETQNKNKPGEGSSVKTEAADQNGNDNENNRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 7
别名 A kinase anchor protein 18 kDa, AKAP 18, AKAP 7 isoforms alpha and beta, AKAP18, AKAP-7 isoforms alpha and beta
计算分子量 81 aa, 9 kDa
观测分子量 11 kDa, 18 kDa, 37 kDa
GenBank蛋白编号BC016927
基因名称 AKAP7
Gene ID (NCBI) 9465
RRIDAB_2226037
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43687
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKAP7, also named as AKAP15 and AKAP18, localize PKA in complexes with CaV1.2 and PLN, respectively. AKAP7 targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. AKAP7 has short isoform AKAP7 alpha and AKAP7 beta with MW 9-15 kDa; long isoform AKAP7 gamma with MW 37-42 kDa. AKAP7alpha was highly expressed only in brain and weakly in lung lysates from WT animals. This antibody can recognize all the isoforms of AKAP7.

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