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AKAP4 Polyclonal antibody

AKAP4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 24986-1-AP

Print datasheet

Synonyms

A kinase anchor protein 4, A kinase anchor protein 82 kDa, AKAP 4, AKAP 82, AKAP4, AKAP82, CT99, FSC1, hAKAP82, HI, p82, PRKA4

验证数据展示

  • WB analysis of human testis using 24986-1-AP

    WB analysis of human testis using 24986-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24986-1-AP (AKAP4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24986-1-AP

    IHC staining of human breast cancer using 24986-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24986-1-AP (AKAP4 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24986-1-AP is AKAP4 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen AKAP4 fusion protein Ag21762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name A kinase (PRKA) anchor protein 4
Synonyms A kinase anchor protein 4, A kinase anchor protein 82 kDa, AKAP 4, AKAP 82, AKAP4, AKAP82, CT99, FSC1, hAKAP82, HI, p82, PRKA4
Calculated Molecular Weight 854 aa, 94 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC126252
Gene Symbol AKAP4
Gene ID (NCBI) 8852
RRIDAB_2876859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JQC9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP4, also named as A-kinase anchor protein 82 kDa, is a 854 amino acid protein, which belongs to the AKAP110 family. AKAP4 localizes to the principle piece of the sperm flagellum and is only expressed in round spermatids. AKAP4 is a major structural component of sperm fibrous sheath and plays a role in sperm motility.

实验方案

Product Specific Protocols
WB protocol for AKAP4 antibody 24986-1-APDownload protocol
IHC protocol for AKAP4 antibody 24986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.

Authors - Tong Liu
View Article
humanIF

Andrologia

Successful outcomes of intracytoplasmic sperm injection-embryo transfer using ejaculated spermatozoa from two Chinese asthenoteratozoospermic brothers with a compound heterozygous FSIP2 mutation.

Authors - Yuan Yuan
View Article