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验证数据展示

  • IHC staining of mouse testis using 66255-1-Ig

    IHC staining of mouse testis using 66255-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 66255-1-Ig

    IHC staining of mouse testis using 66255-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 66255-1-Ig

    IF Staining of mouse testis using 66255-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of human testis using 66255-1-Ig

    WB analysis of human testis using 66255-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66255-1-Ig (AKAP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 66255-1-Ig

    IF Staining of mouse testis using 66255-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

AKAP3 Monoclonal antibody

AKAP3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66255-1-Ig

产品说明书

CloneNo. 3C11H9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66255-1-Ig targets AKAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4885

Product name: Recombinant human AKAP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-353 aa of BC047535

Sequence: MSEKVDWLQSQNGVCKVDVYSPGDNQAQDWKMDTSTDPVRVLSWLRRDLEKSTAEFQDVRFKPGESFGGETSNSGDPHKGFSVDYYNTTTKGTPERLHFEMTHKEIPCQGPRAQLGNESSVDEVSFYANRLTNLVIAMARKEINEKIDGSENKCVYQSLYMGNEPTPTKSLSKIASELVNETVSACSRNAAPDKAPGSGDRVSGSSQSPPNLKYKSTLKIKESTKERQGPDDKPPSKKSFFYKEVFESRNGDYAREGGRFFPRERKRFRGQERPDDFTASVSEGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATILLKKVLLKHAKEVVSDLIDSFLRNLHSVTGTLMTDTQ

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 3
别名 A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein
计算分子量 95 kDa
观测分子量 100 kDa
GenBank蛋白编号BC047535
基因名称 AKAP3
Gene ID (NCBI) 10566
RRIDAB_2881643
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75969
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

实验方案

Product Specific Protocols
IF protocol for AKAP3 antibody 66255-1-IgDownload protocol
IHC protocol for AKAP3 antibody 66255-1-IgDownload protocol
WB protocol for AKAP3 antibody 66255-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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