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AKAP10 Polyclonal antibody

AKAP10 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12356-1-AP

Print datasheet

Synonyms

AKAP 10, AKAP10, D AKAP 2, D AKAP2, PRKA10

验证数据展示

  • WB analysis using 12356-1-AP

    WB analysis using 12356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12356-1-AP (AKAP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12356-1-AP

    IHC staining of human colon cancer using 12356-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12356-1-AP

    IHC staining of human colon cancer using 12356-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12356-1-AP targets AKAP10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen AKAP10 fusion protein Ag3020 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name A kinase (PRKA) anchor protein 10
Synonyms AKAP 10, AKAP10, D AKAP 2, D AKAP2, PRKA10
Calculated Molecular Weight 662 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC017055
Gene Symbol AKAP10
Gene ID (NCBI) 11216
RRIDAB_2225588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43572
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKAP10 antibody 12356-1-APDownload protocol
IHC protocol for AKAP10 antibody 12356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Authors - Ting Peng
View Article