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验证数据展示

  • WB analysis using 17332-1-AP

    WB analysis using 17332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17332-1-AP (AK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

AK7 Polyclonal antibody

AK7 Polyclonal Antibody for WB, ELISA
Cat No. 17332-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

AK 7, ATP-AMP transphosphorylase 7, EC:2.7.4.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue, human testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17332-1-AP targets AK7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11358

Product name: Recombinant human AK7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-502 aa of BC035256

Sequence: SREDLLMRLLECDVIIYNITESSQQMEEAIWAVSALSEEVSHFEKRKLFILLSTVMTWARSKALDPEDSEVPFTEEDYRRRKSHPNFLDHINAEKMVLKFGKKARKFAAYVVAAGLQYGAEGGMLHTFFKMAWLGEIPALPVFGDGTNVIPTIHVLDLAGVIQNVIDHVPKPHYLVAVDESVHTLEDIVKCISKNTGPGKIQKIPRENAYLTKDLTQDCLDHLLVNLRMEALFVKENFNIRWAAQTGFVENINTILKEYKQSRGLMPIKICILGPPAVGKSSIAKELAKYYKLHHIQLKDVISEAIAKLEAIVAPNDVGEGEEEVEEEEEEENVEDAQELLDGIKESMEQNAGQLDDQYIIRFMKEKLKSMPCRNQGYILDGFPKTYDQAKDLFNQEDEEEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenylate kinase 7
别名 AK 7, ATP-AMP transphosphorylase 7, EC:2.7.4.3
计算分子量 656 aa, 75 kDa
观测分子量 83 kDa
GenBank蛋白编号BC035256
基因名称 AK7
Gene ID (NCBI) 122481
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96M32
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AK7 (Adenylate kinase 7) is a nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. AK7 has the highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. It also displays broad nucleoside diphosphate kinase activity and is involved in maintaining ciliary structure and function (PMID: 21080915; 23416111).

实验方案

Product Specific Protocols
WB protocol for AK7 antibody 17332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols