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验证数据展示

  • WB analysis using 12562-1-AP (same clone as 12562-1-PBS)

    WB analysis using 12562-1-AP (same clone as 12562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • WB analysis using 12562-1-AP (same clone as 12562-1-PBS)

    WB analysis using 12562-1-AP (same clone as 12562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12562-1-AP (same clone as 12562-1-PBS)

    IHC staining of human pancreas cancer using 12562-1-AP (same clone as 12562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12562-1-AP (same clone as 12562-1-PBS)

    IF Staining of HepG2 using 12562-1-AP (same clone as 12562-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AK3 antibody (12562-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 12562-1-AP (same clone as 12562-1-PBS)

    IP experiment of mouse kidney using 12562-1-AP (same clone as 12562-1-PBS)

    IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

AK3 Polyclonal antibody, PBS Only

AK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12562-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Adenylate kinase 3 alpha-like 1, AK 3, AK3L1, AK6, AKL3L

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12562-1-PBS targets AK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3146

Product name: Recombinant human AK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-227 aa of BC013771

Sequence: MGASARLLRAVIMGAPGSGKGTVSSRITTHFELKHLSSGDLLRDNMLRGTEIGVLAKAFIDQGKLIPDDVMTRLALHELKNLTQYSWLLDGFPRTLPQAEALDRAYQIDTVINLNVPFEVIKQRLTARWIHPASGRVYNIEFNPPKTVGIDDLTGEPLIQREDDKPETVIKRLKAYEDQTKPVLEYYQKKGVLETFSGTETNKIWPYVYAFLQTKVPQRSQKASVTP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenylate kinase 3
别名 Adenylate kinase 3 alpha-like 1, AK 3, AK3L1, AK6, AKL3L
计算分子量 227 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC013771
基因名称 AK3
Gene ID (NCBI) 50808
RRIDAB_2305373
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIJ7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.

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