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AIF Monoclonal antibody
AIF Monoclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig, Canine and More (2)
Applications
IF/ICC,IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
8C12B2
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells, MDCK cells, Pig brain cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, human kidney tissue, HCT 116 cells |
Planning an IHC experiment? We recommend our IHCeasy AIFM1 Ready-To-Use IHC Kit. AIFM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
67791-1-Ig targets AIF in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Canine samples.
| Tested Applications | IF/ICC,IF-P, IHC, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, Mouse, Rat, Pig, Canine |
| Cited Reactivity | human, rat |
| Immunogen | AIF fusion protein Ag12400 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | apoptosis-inducing factor, mitochondrion-associated, 1 |
| Synonyms | AIF, AIFM1, Cell and organelle markers, PDCD8 |
| Calculated Molecular Weight | 609 aa, 66 kDa |
| Observed Molecular Weight | 67 kDa, 57 kDa |
| GenBank Accession Number | BC111065 |
| Gene Symbol | AIFM1 |
| Gene ID (NCBI) | 9131 |
| RRID | AB_2918555 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95831 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AIF antibody 67791-1-Ig | Download protocol |
| IHC protocol for AIF antibody 67791-1-Ig | Download protocol |
| IF protocol for AIF antibody 67791-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma. | ||
Inflammation Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro | ||
Adv Sci (Weinh) Use of Deep-Learning Assisted Assessment of Cardiac Parameters in Zebrafish to Discover Cyanidin Chloride as a Novel Keap1 Inhibitor Against Doxorubicin-Induced Cardiotoxicity |
