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验证数据展示

  • WB analysis using 67785-1-Ig (same clone as 67785-1-PBS)

    WB analysis using 67785-1-Ig (same clone as 67785-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67785-1-Ig (AHR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHR antibody included
  • IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67785-1-Ig (AHR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHR antibody included
  • IF Staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    IF Staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Blue (DAPI). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 67785-1-Ig (same clone as 67785-1-PBS)

    IF Staining of mouse testis using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67785-1-Ig (same clone as 67785-1-PBS)

    IF Staining of HepG2 using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

AHR Monoclonal antibody, PBS Only

AHR Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67785-1-PBS

产品说明书

CloneNo. 2D1F9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 2D1F9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.

产品信息

67785-1-PBS targets AHR in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28935

Product name: Recombinant human AHR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-300 aa of BC070080

Sequence: PIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQGKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGC

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 aryl hydrocarbon receptor
别名 Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 2D1F9
计算分子量 848 aa, 96 kDa
观测分子量105-110 kDa
GenBank蛋白编号BC070080
基因名称 AHR
Gene ID (NCBI) 196
RRIDAB_2918549
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35869
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

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