• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30242-1-AP

    WB analysis using 30242-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30242-1-AP (AHR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 30242-1-AP

    WB analysis of A549 using 30242-1-AP

    WB result of AHR antibody (30242-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and AHR knockout A549 cells.

  • IHC staining of human ovary cancer using 30242-1-AP

    IHC staining of human ovary cancer using 30242-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30242-1-AP (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AHR antibody included
  • IHC staining of human ovary cancer using 30242-1-AP

    IHC staining of human ovary cancer using 30242-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30242-1-AP (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AHR antibody included
  • Featured Product
  • KD/KO验证

AHR Polyclonal antibody

AHR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30242-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Ah receptor, bHLHe76, Class E basic helix-loop-helix protein 76

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, A549 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30242-1-AP targets AHR in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32335

Product name: Recombinant human AHR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 637-848 aa of BC070080

Sequence: QLCQKMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYPMGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aryl hydrocarbon receptor
别名 Ah receptor, bHLHe76, Class E basic helix-loop-helix protein 76
计算分子量 848 aa, 96 kDa
观测分子量100 kDa
GenBank蛋白编号BC070080
基因名称 AHR
Gene ID (NCBI) 196
RRIDAB_3086275
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35869
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

实验方案

Product Specific Protocols
IHC protocol for AHR antibody 30242-1-APDownload protocol
WB protocol for AHR antibody 30242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...