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AHCY Monoclonal antibody, PBS Only

AHCY Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9B10

Cat No : 66019-1-PBS

Print datasheet

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

验证数据展示

  • WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHCY antibody included
  • IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHCY antibody included
  • IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AHCY Ready-To-Use IHC Kit. AHCY primary antibody included.

产品信息

66019-1-PBS targets AHCY in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF, IHC, Indirect ELISA
Tested Reactivity human
Immunogen AHCY fusion protein Ag18036 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name S-adenosylhomocysteine hydrolase
Synonyms Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC011606
Gene Symbol AHCY
Gene ID (NCBI) 191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23526
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).