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验证数据展示

  • WB analysis using 30923-1-AP (same clone as 30923-1-PBS)

    WB analysis using 30923-1-AP (same clone as 30923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30923-1-AP (AHCTF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30923-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 30923-1-AP (same clone as 30923-1-PBS)

    IF Staining of A431 using 30923-1-AP (same clone as 30923-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using AHCTF1 antibody (30923-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 30923-1-PBS in a different storage buffer formulation.

AHCTF1 Polyclonal antibody, PBS Only

AHCTF1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30923-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ELYS, TMBS62, MSTP108, MST108

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AHCTF1 Ready-To-Use IHC Kit. AHCTF1 primary antibody included.

产品信息

30923-1-PBS targets AHCTF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32788

Product name: Recombinant human AHCTF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2067-2266 aa of NM_001323342.1

Sequence: ERTDEMTHKETNEQEERLLATASFTKSSRSSRTRSSKAILLPDLSEPNNEPLFSPASEVPRKAKAKKIEVPAQLKELVSDLSSQFVISPPALRSRQKNTSNKNKLEDELKDDAQSVETLGKPKAKRIRTSKTKQASKNTEKESAWSPPPIEIRLISPLASPADGVKSKPRKTTEVTGTGLGRNRKKLSSYPKQILRRKML

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AT hook containing transcription factor 1
别名 ELYS, TMBS62, MSTP108, MST108
计算分子量252 kDa
观测分子量270 kDa
GenBank蛋白编号NM_001323342.1
基因名称 AHCTF1
Gene ID (NCBI) 25909
RRIDAB_3669786
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYP5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AHCTF1, also known as ELYS, TMBS62, is a large multifunctional protein. It plays essential roles in nuclear pore assembly and mitosis. AHCTF1 was expressed at higher levels in embryonic liver, spleen and thymus compared to the corresponding adult tissues (PMID: 11952839) and has 3 isoforms with MW of 252~256 kDa.

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