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验证数据展示

  • WB analysis using 16449-1-AP

    WB analysis using 16449-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16449-1-AP (AGXT2L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 16449-1-AP

    IHC staining of human prostate cancer using 16449-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16449-1-AP (AGXT2L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 16449-1-AP

    IHC staining of human prostate cancer using 16449-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16449-1-AP (AGXT2L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AGXT2L2 Polyclonal antibody

AGXT2L2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16449-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PHYKPL, 5-phosphohydroxy-L-lysine phospho-lyase, Alanine--glyoxylate aminotransferase 2-like 2, EC:4.2.3.134, PP9286

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16449-1-AP targets AGXT2L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8391

Product name: Recombinant human AGXT2L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 276-450 aa of BC008009

Sequence: MGKSIGNGHPVACVAATQPVARAFEATGVEYFNTFGGSPVSCAVGLAVLNVLEKEQLQDHATSVGSFLMQLLGQQKIKHPIVGDVRGVGLFIGVDLIKDEATRTPATEEAAYLVSRLKENYVLLSTDGPGRNILKFKPPMCFSLDNARQVVAKLDAILTDMEEKVRSCETLRLQP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alanine-glyoxylate aminotransferase 2-like 2
别名 PHYKPL, 5-phosphohydroxy-L-lysine phospho-lyase, Alanine--glyoxylate aminotransferase 2-like 2, EC:4.2.3.134, PP9286
计算分子量450aa,50 kDa; 175aa,19 kDa
观测分子量50 kDa
GenBank蛋白编号BC008009
基因名称 AGXT2L2
Gene ID (NCBI) 85007
RRIDAB_2225868
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUZ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGXT2L2, also known as alanine-glyoxylate aminotransferase 2-like 2 or PHYKPL (5-phosphohydroxy-L-lysine phospho-lyase), is a nuclear gene encoding a mitochondrial enzyme. It belongs to the class-III pyridoxal phosphate-dependent aminotransferase family and shares approximately 36% sequence identity with AGXT2.

实验方案

Product Specific Protocols
WB protocol for AGXT2L2 antibody 16449-1-APDownload protocol
IHC protocol for AGXT2L2 antibody 16449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols