验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | mouse brain tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17042-1-AP targets AGXT2L1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag10584 Product name: Recombinant human AGXT2L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 147-499 aa of BC022526 Sequence: IEISPYKFQKGKDVKKEFVHVAPTPDTYRGKYREDHADSASAYADEVKKIIEDAHNSGRKIAAFIAESMQSCGGQIIPPAGYFQKVAEYVHGAGGVFIADEVQVGFGRVGKHFWSFQMYGEDFVPDIVTMGKPMGNGHPVACVVTTKEIAEAFSSSGMEYFNTYGGNPVSCAVGLAVLDIIENEDLQGNAKRVGNYLTELLKKQKAKHTLIGDIRGIGLFIGIDLVKDHLKRTPATAEAQHIIYKMKEKRVLLSADGPHRNVLKIKPPMCFTEEDAKFMVDQLDRILTVLEEAMGTKTESVTSENTPCKTKMLKEAHIELLRDSTTDSKENPSRKRNGMCTDTHSLLSKRLKT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | alanine-glyoxylate aminotransferase 2-like 1 |
| 别名 | AGXT2L1, ETNPPL |
| 计算分子量 | 499 aa, 56 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC022526 |
| 基因名称 | AGXT2L1 |
| Gene ID (NCBI) | 64850 |
| RRID | AB_3085514 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TBG4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), also called ethanolamine phosphate phospholyase (ETNPPL), is a brain enzyme, so-named after its similarity to AGXT2 (36% sequence identity), that may be involved in the pathogenesis of severe neuropsychiatric disturbances(PMID: 23761375). AGXT2L1 is significantly down-regulated in cancerous tissues and may be a tumor suppressor(PMID: 26294768, 32724374).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for AGXT2L1 antibody 17042-1-AP | Download protocol |
| WB protocol for AGXT2L1 antibody 17042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
