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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

    WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

    WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83385-6-RR (same clone as 83385-6-PBS)

    FC experiment of HepG2 using 83385-6-RR (same clone as 83385-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AGXT Recombinant antibody (83385-6-RR, Clone:240305E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83385-6-RR (same clone as 83385-6-PBS)

    IF Staining of HeLa using 83385-6-RR (same clone as 83385-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AGXT antibody (83385-6-RR, Clone: 240305E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83385-6-RR

    Affinity and Kinetic Characterization of 83385-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83385-6-RR against Human AGXT were performed. The affinity constant is 0.226 nM.

AGXT Recombinant antibody, PBS Only

AGXT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83385-6-PBS

产品说明书

CloneNo. 240305E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.

产品信息

83385-6-PBS targets AGXT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 AGXT fusion protein Ag18062 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alanine-glyoxylate aminotransferase
别名 EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT
计算分子量 392 aa, 43 kDa
GenBank蛋白编号BC132819
基因名称 AGXT
Gene ID (NCBI) 189
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP21549
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C