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验证数据展示

  • WB analysis using 67402-1-Ig (same clone as 67402-1-PBS)

    WB analysis using 67402-1-Ig (same clone as 67402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67402-1-Ig (same clone as 67402-1-PBS)

    IHC staining of human liver cancer using 67402-1-Ig (same clone as 67402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGXT antibody included
  • IHC staining of human kidney using 67402-1-Ig (same clone as 67402-1-PBS)

    IHC staining of human kidney using 67402-1-Ig (same clone as 67402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGXT antibody included

AGXT Monoclonal antibody, PBS Only

AGXT Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67402-1-PBS

产品说明书

CloneNo. 3E12H1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

3E12H1, AGT, AGT1, AGXT1, Alanine--glyoxylate aminotransferase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.

产品信息

67402-1-PBS targets AGXT in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19188

Product name: Recombinant human AGXT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-392 aa of BC132819

Sequence: MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alanine-glyoxylate aminotransferase
别名 3E12H1, AGT, AGT1, AGXT1, Alanine--glyoxylate aminotransferase
计算分子量 392 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC132819
基因名称 AGXT
Gene ID (NCBI) 189
RRIDAB_2882644
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21549
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

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