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验证数据展示

  • WB analysis using 20692-1-AP (same clone as 20692-1-PBS)

    WB analysis using 20692-1-AP (same clone as 20692-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20692-1-AP (AGRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20692-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 20692-1-AP (same clone as 20692-1-PBS)

    IHC staining of human lung using 20692-1-AP (same clone as 20692-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20692-1-AP (AGRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20692-1-PBS in a different storage buffer formulation.

  • IF Staining of Saos-2 using 20692-1-AP (same clone as 20692-1-PBS)

    IF Staining of Saos-2 using 20692-1-AP (same clone as 20692-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using AGRN antibody (20692-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20692-1-PBS in a different storage buffer formulation.

AGRN Polyclonal antibody, PBS Only

AGRN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20692-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Agrin, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 22 kDa fragment, Agrin C-terminal 90 kDa fragment, Agrin N-terminal 110 kDa subunit

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20692-1-PBS targets AGRN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 agrin
别名 Agrin, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 22 kDa fragment, Agrin C-terminal 90 kDa fragment, Agrin N-terminal 110 kDa subunit
计算分子量 215 kDa
观测分子量225 kDa, 97 kDa
GenBank蛋白编号NM_198576
基因名称 AGRN
Gene ID (NCBI) 375790
RRIDAB_3085612
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00468
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Agrin, a large heparan sulfate proteoglycan, is expressed in neuronal, as well as nonneuronal tissues (PMID:12671652). Nerve-derived agrin has been demonstrated to play an essential role in development and maintenance of the neuromuscular junction (PMID:16386415). The agrin gene encodes a protein of more than 2000 amino acids with predicted molecular weight of 225 kDa but can display as 600 kDa protein due to extensive N-terminal glycosylation (PMID:12671652). Neurotrypsin cleaves the proteoglycan agrin at two homologous, highly conserved sites, liberating a middle 90-kDa and a C-terminal 22-kDa fragment from the membrane or ECM-bound N-terminal moiety of agrin (PMID:18230682).

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