AGR3 Polyclonal antibody
AGR3 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SKOV-3 cells |
Planning an IHC experiment? We recommend our IHCeasy AGR3 Ready-To-Use IHC Kit. AGR3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11967-1-AP targets AGR3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, WB,ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, chicken |
| Immunogen | AGR3 fusion protein Ag2577 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | anterior gradient homolog 3 (Xenopus laevis) |
| Synonyms | AG 3, AG3, AGR3, hAG 3, HAG3, PDIA18 |
| Calculated Molecular Weight | 166 aa, 19 kDa |
| Observed Molecular Weight | 19-25 kDa |
| GenBank Accession Number | BC058284 |
| Gene Symbol | AGR3 |
| Gene ID (NCBI) | 155465 |
| RRID | AB_2877809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TD06 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AGR3 antibody 11967-1-AP | Download protocol |
| IHC protocol for AGR3 antibody 11967-1-AP | Download protocol |
| IF protocol for AGR3 antibody 11967-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer. | ||
Front Pharmacol AGR3 Regulates Airway Epithelial Junctions in Patients with Frequent Exacerbations of COPD. | ||
iScience Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium | ||
J Transl Med Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers |
