验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human testis tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
16762-1-AP targets AGPAT6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag10294 Product name: Recombinant human AGPAT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 206-456 aa of BC051377 Sequence: KEFMSKHVHLMCYRICVRALTAIITYHDRENRPRNGGICVANHTSPIDVIILASDGYYAMVGQVHGGLMGVIQRAMVKACPHVWFERSEVKDRHLVAKRLTEHVQDKSKLPILIFPEGTCINNTSVMMFKKGSFEIGATVYPVAIKYDPQFGDAFWNSSKYGMVTYLLRMMTSWAIVCSVWYLPPMTREADEDAVQFANRVKSAIARQGGLVDLLWDGGLKREKVKDTFKEEQQKLYSKMIVGNHKDRSRS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta) |
| 别名 | 1 AGP acyltransferase 6, 1 AGPAT 6, AGPAT6, GPAT4, LPAAT zeta, LPAATZ, TSARG7 |
| 计算分子量 | 456 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC051377 |
| 基因名称 | AGPAT6 |
| Gene ID (NCBI) | 137964 |
| RRID | AB_1850878 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86UL3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family. AGPAT6 is expressed in brown adipose tissue and mammary epithelium as well as many other tissues. A deficiency of AGPAT6 in mice results in resistance to dietinduced and genetic forms of obesity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AGPAT6 antibody 16762-1-AP | Download protocol |
| IHC protocol for AGPAT6 antibody 16762-1-AP | Download protocol |
| IP protocol for AGPAT6 antibody 16762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves. | ||
EMBO Rep MTCH2 cooperates with MFN2 and lysophosphatidic acid synthesis to sustain mitochondrial fusion |
