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验证数据展示

  • WB analysis using 21899-1-AP

    WB analysis using 21899-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21899-1-AP (AGMAT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 21899-1-AP

    IHC staining of human stomach cancer using 21899-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21899-1-AP (AGMAT antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AGMAT Polyclonal antibody

AGMAT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21899-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Agmatinase, Arginase, mitochondrial, Agmatine ureohydrolase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21899-1-AP targets AGMAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AGMAT fusion protein Ag13609 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 agmatine ureohydrolase (agmatinase)
别名 Agmatinase, Arginase, mitochondrial, Agmatine ureohydrolase
计算分子量 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC005090
基因名称 AGMAT
Gene ID (NCBI) 79814
RRIDAB_3085677
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BSE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGMAT is a metallohydrolase and belongs to the arginase family. AGMAT plays an important role in amino acid metabolism and can hydrolyze agmatine into urea and putrescine. AGMAT has been shown to play an important role in the development of tumors, including colorectal cancer, lung adenocarcinoma, and pancreatic adenocarcinoma(PMID: 36680755; PMID: 31699997; PMID: 35837051 ).

实验方案

Product Specific Protocols
WB protocol for AGMAT antibody 21899-1-APDownload protocol
IHC protocol for AGMAT antibody 21899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols