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验证数据展示

  • WB analysis using 86187-1-RR

    WB analysis using 86187-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86187-1-RR

    WB analysis using 86187-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86187-1-RR

    Affinity and Kinetic Characterization of 86187-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86187-1-RR against Human AGL were performed. The affinity constant is below 1 pM.

AGL Recombinant monoclonal antibody

AGL Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86187-1-RR

产品说明书

CloneNo. 250767A6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, Jurkat cells, K-562 cells, rat skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86187-1-RR targets AGL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9962

Product name: Recombinant human AGL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1182-1533 aa of BC078663

Sequence: MYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
别名 4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher
计算分子量 1532 aa, 175 kDa
观测分子量 165 kDa
GenBank蛋白编号BC078663
基因名称 AGL
Gene ID (NCBI) 178
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35573
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.

实验方案

Product Specific Protocols
WB protocol for AGL antibody 86187-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols