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验证数据展示

  • WB analysis using 86187-1-RR (same clone as 86187-1-PBS)

    WB analysis using 86187-1-RR (same clone as 86187-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86187-1-PBS in a different storage buffer formulation.

  • WB analysis using 86187-1-RR (same clone as 86187-1-PBS)

    WB analysis using 86187-1-RR (same clone as 86187-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86187-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86187-1-RR

    Affinity and Kinetic Characterization of 86187-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86187-1-RR against Human AGL were performed. The affinity constant is below 1 pM.

AGL Recombinant monoclonal antibody, PBS Only

AGL Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86187-1-PBS

产品说明书

CloneNo. 250767A6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86187-1-PBS targets AGL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9962

Product name: Recombinant human AGL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1182-1533 aa of BC078663

Sequence: MYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
别名 4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher
计算分子量 1532 aa, 175 kDa
观测分子量 165 kDa
GenBank蛋白编号BC078663
基因名称 AGL
Gene ID (NCBI) 178
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35573
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.

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