- Featured Product
- KD/KO Validated
AGL Polyclonal antibody
AGL Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, human, rat
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse heart tissue, HEK-293 cells, rat heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
16582-1-AP targets AGL in WB, IHC, ELISA applications and shows reactivity with mouse, human, rat samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | mouse, human, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | AGL fusion protein Ag9962 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | amylo-1, 6-glucosidase, 4-alpha-glucanotransferase |
| Synonyms | AGL, Amylo 1,6 glucosidase, Dextrin 6 alpha D glucosidase, GDE, Glycogen debranching enzyme |
| Calculated Molecular Weight | 1532 aa, 175 kDa |
| Observed Molecular Weight | 165 kDa |
| GenBank Accession Number | BC078663 |
| Gene Symbol | AGL |
| Gene ID (NCBI) | 178 |
| RRID | AB_2878283 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35573 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AGL antibody 16582-1-AP | Download protocol |
| IHC protocol for AGL antibody 16582-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III | ||
iScience MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes. | ||
PLoS One A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise | ||
Stem Cell Res Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III |
