验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84715-4 targets AGGF1 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag2497 Product name: Recombinant human AGGF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-270 aa of BC029382 Sequence: DMEHTLRIPEVGVSKFHAEIYFDHDLQSYVPVDQGSQNGTIVNGKQILQPKTKCDPYVLEHGDEVKIGETVLSFHIHPGSDTCDGCEPGQVRAHLRLDKKDESFVGPTLSKEEKELERRKELKKIRVKYGLQNTEYEDEKTLKNPKYKDRAGKRREQVGSEGTFQRDDAPASVHSEITDSNKGRKMLEKMGWKKGEGLGKDGGGMKTPIQLQLRRTHAGLGTGKPSSFEDVHLLQNKNKKNWDKARERFTENFPETKPQKDDPGTMP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | angiogenic factor with G patch and FHA domains 1 |
| 别名 | 242264F4, Angiogenic factor VG5Q, G patch domain-containing protein 7, GPATC7, GPATCH7 |
| 计算分子量 | 714 aa, 81 kDa |
| GenBank蛋白编号 | BC029382 |
| 基因名称 | AGGF1 |
| Gene ID (NCBI) | 55109 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N302 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AGGF1 antibody CL488-84715-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |