验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, HeLa cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11889-1-AP targets AGGF1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish, macaque, rhesus |
| 免疫原 |
CatNo: Ag2497 Product name: Recombinant human AGGF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-270 aa of BC029382 Sequence: DMEHTLRIPEVGVSKFHAEIYFDHDLQSYVPVDQGSQNGTIVNGKQILQPKTKCDPYVLEHGDEVKIGETVLSFHIHPGSDTCDGCEPGQVRAHLRLDKKDESFVGPTLSKEEKELERRKELKKIRVKYGLQNTEYEDEKTLKNPKYKDRAGKRREQVGSEGTFQRDDAPASVHSEITDSNKGRKMLEKMGWKKGEGLGKDGGGMKTPIQLQLRRTHAGLGTGKPSSFEDVHLLQNKNKKNWDKARERFTENFPETKPQKDDPGTMP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | angiogenic factor with G patch and FHA domains 1 |
| 别名 | HUS84971, HSU84971, GPATCH7, GPATC7, G patch domain-containing protein 7 |
| 计算分子量 | 714 aa, 81 kDa |
| 观测分子量 | 84-100 kDa |
| GenBank蛋白编号 | BC029382 |
| 基因名称 | AGGF1 |
| Gene ID (NCBI) | 55109 |
| RRID | AB_2305338 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N302 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AGGF1 antibody 11889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure. | ||
Nat Commun AGGF1 therapy inhibits thoracic aortic aneurysms by enhancing integrin α7-mediated inhibition of TGF-β1 maturation and ERK1/2 signaling | ||
Nat Commun Endothelial AGGF1 promotes retinal angiogenesis by coordinating TNFSF12/FN14 signalling
| ||
Mol Ther The 3' Untranslated Region Protects the Heart from Angiotensin II-Induced Cardiac Dysfunction via AGGF1 Expression. | ||
Arterioscler. Thromb. Vasc. Biol. Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury.
| ||
Arterioscler Thromb Vasc Biol Aggf1 Specifies Hemangioblasts at the Top of Regulatory Hierarchy via Npas4l and mTOR-S6K-Emp2-ERK Signaling
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