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验证数据展示

  • WB analysis of Jurkat using 11919-1-AP

    WB analysis of Jurkat using 11919-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11919-1-AP

    IHC staining of human lymphoma using 11919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11919-1-AP (AGFG2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 11919-1-AP

    IF Staining of HepG2 using 11919-1-AP

    Immunofluorescent analysis of HepG2 cells, using AGFG2 antibody 11919-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

AGFG2 Polyclonal antibody

AGFG2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11919-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

RABR, RAB R, HRBL, HIV-1 Rev-binding protein-like protein, ArfGAP with FG repeats 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11919-1-AP targets AGFG2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2546

Product name: Recombinant human AGFG2 protein

Source: e coli.-derived, T-GST

Tag: GST

Domain: 1-300 aa of BC017329

Sequence: MVMAAKKGPGPGGGVSGGKAEAEAASEVWCRRVRELGGCSQAGNRHCFECAQRGVTYVDITVGSFVCTTCSGLLRGLNPPHRVKSISMTTFTEPEVVFLQSRGNEVCRKIWLGLFDARTSLVPDSRDPQKVKEFLQEKYEKKRWYVPPDQVKGPTYTKGSASTPVQGSIPEGKPLRTLLGDPAPSLSVAASTSSQPVSQSHARTSQARSTQPPPHSSVKKASTDLLADIGGDPFAAPQMAPAFAAFPAFGGQTPSQGGFANFDAFSSGPSSSVFGSLPPAGQASFQAQPTPAGSSQGTPF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ArfGAP with FG repeats 2
别名 RABR, RAB R, HRBL, HIV-1 Rev-binding protein-like protein, ArfGAP with FG repeats 2
计算分子量 481 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC017329
基因名称 AGFG2
Gene ID (NCBI) 3268
RRIDAB_2224646
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95081
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for AGFG2 antibody 11919-1-APDownload protocol
IHC protocol for AGFG2 antibody 11919-1-APDownload protocol
WB protocol for AGFG2 antibody 11919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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